human ventricular
Cardiac Anisotropic Sheet (hvCAS)
Multicellular visual approach for quantifying risk towards
cardiac electrical disturbances
CUSTOM-DESIGNED SUBSTRATE

Proprietary design to reproduce electrical conduction pattern of native heart.

DIRECT MEASUREMENT

Offers unprecedented direct visualization and measurement of cardiac arrhythmias.

ARRHYTHMIC RISK

Arrhythmic risk can be quantified using hvCAS under drug treatment or disease conditions.

Traditional in vitro electrophysiological measurements are limited to single-cell data that fail to reveal cell-to-cell properties, such as conduction and re-entrant waves or to recapitulate physiological cardiac properties, such as cellular alignment and anisotropic conduction.

Novoheart's human ventricular Cardiac Anisotropic Sheet (hvCAS) model is the first validated multi-cellular optical approach for quantifying risk towards cardiac arrhythmias. Our proprietary microfabricated substrate aligns stem cell-derived cardiomyocytes to mimic structural and functional anisotropic properties of the human heart.

The hvCAS platform is available as two options:

  • (Cat. No. 2-01) Microfabricated polystyrene substrate (Circular disc with 1.5cm diameter, with 8-µm, 10-µm or 15-µm grooves for increasing levels of cell anisotropy)
    8/10/15 µm width x 5 µm depth and 5 µm ridge
  • (Cat. No. 2-02) Microfabricated polystyrene substrate with custom dimensions
  • Cardiac Toxicity Screening: identification of pro-arrhythmic compounds, chronotropes and dromotropes.
  • Disease Modeling: studies on human channelopathies.
  • Drug Discovery: drug screening and efficacy assessment of pro-arrhythmic compounds, chronotropes and dromotropes.
  • Regenerative Studies: studies on electrophysiological maturation in human cardiomyocytes.

Shum, A. M. Y., Che, H., Wong, A. O., Zhang, C., Wu, H., Chan, C. W. Y., Costa, K., Khine, M., Kong, C. & Li, R. A. A Micropatterned Human Pluripotent Stem Cell-Based Ventricular Cardiac Anisotropic Sheet for Visualizing Drug-Induced Arrhythmgencicity. Adanced Mater. 29, (2017).
 

Chen, A., Lee, E., Tu, R., Santiago, K., Grosberg, A., Fowlkes, C. & Khine, M. Integrated platform for functional monitoring of biomimetic heart sheets derived from human pluripotent stem cells. Biomaterials 35, 675–83 (2014).
 

Wang, J., Chen, A., Lieu, D. K., Karakikes, I., Chen, G., Keung, W., Chan, C. W., Hajjar, R. J., Costa, K. D., Khine, M. & Li, R. A. Effect of engineered anisotropy on the susceptibility of human pluripotent stem cell-derived ventricular cardiomyocytes to arrhythmias. Biomaterials 34, 8878–8886 (2013).
 

Chen, A., Lieu, D. K., Freschauf, L., Lew, V., Sharma, H., Wang, J., Nguyen, D., Karakikes, I., Hajjar, R. J., Gopinathan, A., Botvinick, E., Fowlkes, C. C., Li, R. A. & Khine, M. Shrink-film configurable multiscale wrinkles for functional alignment of human embryonic stem cells and their cardiac derivatives. Adv. Mater. 23, 5785–91 (2011).
 

Luna, J. I., Ciriza, J., Garcia-ojeda, M. E., Kong, M., Herren, A., Lieu, D. K., Li, R. A., Fowlkes, C. C., Khine, M. & McCloskey, K. E. Multiscale Biomimetic Topography for the Alignment of Neonatal and Embryonic Stem Cell-Derived Heart Cells. Tissue Eng. Part C Methods 17, 579–588 (2011).


Xue, T., Cho, H. C., Akar, F. G., Tsang, S. Y., Jones, S. P., Marbán, E., Tomaselli, G. F., Li, R. A. Functional integration of electrically active cardiac derivatives from genetically engineered human embryonic stem cells with quiescent recipient ventricular cardiomyocytes: insights into the development of cell-based pacemakers. Circulation. 111, 11-20 (2005). 

 

Product ID Product Name Qty
2-0101-8UM Microfabricated polystyrene substrate 8 µm (Circular disc with 1.5cm diameter)
8 µm width x 5 µm depth and 5 µm ridge
10 Request Quote
2-0102-8UM Microfabricated polystyrene substrate 8 µm (Circular disc with 1.5cm diameter)
8 µm width x 5 µm depth and 5 µm ridge
50 Request Quote
2-0103-10UM Microfabricated polystyrene substrate 10 µm (Circular disc with 1.5cm diameter)
10 µm width x 5 µm depth and 5 µm ridge
10 Request Quote
2-0104-10UM Microfabricated polystyrene substrate 10 µm (Circular disc with 1.5cm diameter)
10 µm width x 5 µm depth and 5 µm ridge
50 Request Quote
2-0105-15UM Microfabricated polystyrene substrate 15 µm (Circular disc with 1.5cm diameter)
15 µm width x 5 µm depth and 5 µm ridge
10 Request Quote
2-0106-15UM Microfabricated polystyrene substrate 15 µm (Circular disc with 1.5cm diameter)
15 µm width x 5 µm depth and 5 µm ridge
50 Request Quote
2-0201 Microfabricated polystyrene substrate with custom dimensions (Circular disc with 1.5cm diameter)
Customized width x 5 µm depth and 5 µm ridge
10 Request Quote
2-0202 Microfabricated polystyrene substrate with custom dimensions (Circular disc with 1.5cm diameter)
Customized width x 5 µm depth and 5 µm ridge
50 Request Quote

Is coating of the microfabricated polystyrene substrates needed?
Substrates should be coated with hESC-qualified Matrigel before cell seeding.

Are the microfabricated polystyrene substrates reusable?
Microfabricated polystyrene substrates are intended for single use.



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